DescriptionHybridization and denaturation transitions between DNA duplex and single stranded forms will be studied using Monte Carlo molecular simulation and a coarse-grained model. Short oligomers, between 10 and 25 bases in length, will be studied for one to two sequences, for cases where both strands are in solution as well as where one strand is bound to a surface as a model for a DNA microarray. The effects of temperature and of surface binding density will also be studied, and the results analyzed in terms of structural effects and hydrogen bonding patterns within the duplex.
OrganizationUniversity of New England
DepartmentChemistry and Physics
Sponsor Campus GridOSG-XSEDE
Principal Investigator
John Stubbs
Field Of ScienceChemistry