errorstudy

Nameerrorstudy
DescriptionMissing data and genotyping errors are common features of microsatellite data sets used to infer the genetic structure of natural populations. We used simulated data to quantify the effect of these data aberrations on the accuracy of population structure inference. Data sets were simulated under the coalescent and ranged from panmictic to highly subdivided with complex, randomly generated, population histories. Models describing the characteristic patterns of missing data and genotyping error in real microsatellite data sets were developed, and used to modify the simulated data sets. Performance of an ordination, a tree based, and a model based Bayesian method of population structure inference was evaluated before and after data set modifications. The ability to recover correct population clusters decreased as missing data increased. The rate of decrease was similar among analytical procedures, thus no single analytical approach was preferable when faced with incomplete data. Researchers should expect to retrieve 3–4% fewer correct clusters for every 1% of a data matrix made up of missing data using these methods. For every 1% of a matrix that contained erroneous genotypes, approximately 1–2% fewer correct clusters were recovered using ordination and tree based methods. A Bayesian procedure that minimizes the deviation from Hardy Weinberg equilibrium in order to assign individuals to clusters performed better as genotyping error increased. We attribute this surprising result to the inbreeding like nature of microsatellite genotyping error, and recommend the use of related analytical methods that explicitly account for inbreeding, as a means to mitigate the effect of genotyping error.
OrganizationUSDA Agricultural Research Service
DepartmentNational Center for Genetic Resources Preservation
Sponsor Campus GridOSG Connect
Principal Investigator
Christopher Richards
Field Of ScienceMolecular and Structural Biosciences
DisableFalse